Next Generation Sequencing (NGS) in Forensic Genetics and Anthropology Training Course

Introduction to NGS and Its Forensic Utility

• Overview of modern DNA sequencing technologies
• Benefits of NGS compared to conventional forensic methods
• Primary forensic applications of NGS

Core Concepts in Forensic Genetics

• Key DNA markers employed in forensic analysis
• Short Tandem Repeats (STRs) and Single Nucleotide Polymorphisms (SNPs)
• Analysis of Mitochondrial DNA and the Y-chromosome

NGS Workflows in Forensic Investigations

• Sample preparation and library construction procedures
• Sequencing protocols specific to forensic samples
• Quality control measures and contamination mitigation

Bioinformatics and Analysis of NGS Data

• Processing and aligning NGS data
• Variant calling and its interpretation in forensic contexts
• Essential software tools for forensic genomic analysis

Forensic Anthropology and Ancestry Profiling

• Genetic markers used for ancestry and population studies
• NGS applications in analyzing ancient and degraded DNA
• Case studies highlighting forensic anthropology

NGS for Complex and Degraded Samples

• Methods for handling low-quality and low-quantity DNA
• Challenges involving environmental and mixed DNA samples
• Application of NGS in missing persons and disaster victim identification

Legal, Ethical, and Regulatory Frameworks

• Privacy issues within forensic genomics
• Regulatory standards for forensic DNA databases
• Ethical dilemmas in forensic genetic analysis

Emerging Trends in Forensic Genomics

• Recent advancements in sequencing technology
• The role of AI in forensic genetics
• Developing forensic applications of NGS

Conclusion and Recommended Next Steps